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hmgb1 protein levels  (Elabscience Biotechnology)


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    Elabscience Biotechnology hmgb1 protein levels
    Hmgb1 Protein Levels, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hmgb1+protein+levels/pm41380410-211-0-17?v=Elabscience+Biotechnology
    Average 93 stars, based on 3 article reviews
    hmgb1 protein levels - by Bioz Stars, 2026-07
    93/100 stars

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    DSF/Cu induces ICD and promotes DC cell activation in HCC. ( A ) Membrane exposure of CRT on HCC cells after treatment with DSF/Cu was detected by flow cytometry. ( B ) Soluble <t>HMGB1</t> in the media of HCC cells treated with DSF/Cu at different concentrations. ( C ) Extracellular ATP in the media of HCC treated with DSF/Cu at indicated concentration. ( D ) Gene-set enrichment analysis of type I interferon response- and inflammatory response-related genes. ( E ) Heatmap of type I interferon response-related genes. ( F – I ) The expression of CFSE, I-Ab, CD80, and CD86 in CD11c + DC cells cocultured with Hepa1–6 cells treated with DSF/Cu was detected by flow cytometry. *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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    DSF/Cu induces ICD and promotes DC cell activation in HCC. ( A ) Membrane exposure of CRT on HCC cells after treatment with DSF/Cu was detected by flow cytometry. ( B ) Soluble <t>HMGB1</t> in the media of HCC cells treated with DSF/Cu at different concentrations. ( C ) Extracellular ATP in the media of HCC treated with DSF/Cu at indicated concentration. ( D ) Gene-set enrichment analysis of type I interferon response- and inflammatory response-related genes. ( E ) Heatmap of type I interferon response-related genes. ( F – I ) The expression of CFSE, I-Ab, CD80, and CD86 in CD11c + DC cells cocultured with Hepa1–6 cells treated with DSF/Cu was detected by flow cytometry. *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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    Figure 1. Schematic description of serum <t>HMGB1</t> levels in patients and controls.
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    DSF/Cu induces ICD and promotes DC cell activation in HCC. ( A ) Membrane exposure of CRT on HCC cells after treatment with DSF/Cu was detected by flow cytometry. ( B ) Soluble HMGB1 in the media of HCC cells treated with DSF/Cu at different concentrations. ( C ) Extracellular ATP in the media of HCC treated with DSF/Cu at indicated concentration. ( D ) Gene-set enrichment analysis of type I interferon response- and inflammatory response-related genes. ( E ) Heatmap of type I interferon response-related genes. ( F – I ) The expression of CFSE, I-Ab, CD80, and CD86 in CD11c + DC cells cocultured with Hepa1–6 cells treated with DSF/Cu was detected by flow cytometry. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

    Journal: Cancers

    Article Title: Disulfiram/Copper Induces Immunogenic Cell Death and Enhances CD47 Blockade in Hepatocellular Carcinoma

    doi: 10.3390/cancers14194715

    Figure Lengend Snippet: DSF/Cu induces ICD and promotes DC cell activation in HCC. ( A ) Membrane exposure of CRT on HCC cells after treatment with DSF/Cu was detected by flow cytometry. ( B ) Soluble HMGB1 in the media of HCC cells treated with DSF/Cu at different concentrations. ( C ) Extracellular ATP in the media of HCC treated with DSF/Cu at indicated concentration. ( D ) Gene-set enrichment analysis of type I interferon response- and inflammatory response-related genes. ( E ) Heatmap of type I interferon response-related genes. ( F – I ) The expression of CFSE, I-Ab, CD80, and CD86 in CD11c + DC cells cocultured with Hepa1–6 cells treated with DSF/Cu was detected by flow cytometry. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

    Article Snippet: The supernatants of cells cultured under the conditions described above were evaluated for extracellular HMGB1 levels using an ELISA kit (Cusabio, CSB-E08223h and CSB-E08225m, Wuhan, China).

    Techniques: Activation Assay, Membrane, Flow Cytometry, Concentration Assay, Expressing

    Inhibition of NPL4 inhibits proliferation and induces ICD in HCC cells. ( A ) Inhibition efficiency of NPL4 in LM3 cells through Western blot analysis. ( B ) The role of NPL4 inhibition in cell viability was detected by CCK-8 assay. ( C ) The role of NPL4 inhibition in clone-forming capacity of LM3 cells. ( D ) The role of NPL4 inhibition in cell proliferation was detected by Edu assay. Images with magnification at 100× are shown here. ( E ) Apoptosis analysis of LM3 cells after NPL4 inhibition. ( F ) CRT exposure, ( G ) HMGB1 release, and ( H ) ATP secretion of LM3 cells after NPL4 inhibition. ( I ) Western blot analysis of unfolded protein response and poly-ubiquitylated proteins in LM3 cells after NPL4 inhibition. ( J ) Pathway analysis of LM3 cells after NPL4 inhibition. ( K ) Gene-set enrichment analysis of type Ⅰ interferon response- and interferon-gamma response-related genes in LM3 cells after NPL4 inhibition. ( L ) Heatmap of type Ⅰ interferon response-related genes in LM3 cells after NPL4 inhibition. *: p < 0.05; **, p < 0.01; ***, p < 0.001. The uncropped blots are shown in .

    Journal: Cancers

    Article Title: Disulfiram/Copper Induces Immunogenic Cell Death and Enhances CD47 Blockade in Hepatocellular Carcinoma

    doi: 10.3390/cancers14194715

    Figure Lengend Snippet: Inhibition of NPL4 inhibits proliferation and induces ICD in HCC cells. ( A ) Inhibition efficiency of NPL4 in LM3 cells through Western blot analysis. ( B ) The role of NPL4 inhibition in cell viability was detected by CCK-8 assay. ( C ) The role of NPL4 inhibition in clone-forming capacity of LM3 cells. ( D ) The role of NPL4 inhibition in cell proliferation was detected by Edu assay. Images with magnification at 100× are shown here. ( E ) Apoptosis analysis of LM3 cells after NPL4 inhibition. ( F ) CRT exposure, ( G ) HMGB1 release, and ( H ) ATP secretion of LM3 cells after NPL4 inhibition. ( I ) Western blot analysis of unfolded protein response and poly-ubiquitylated proteins in LM3 cells after NPL4 inhibition. ( J ) Pathway analysis of LM3 cells after NPL4 inhibition. ( K ) Gene-set enrichment analysis of type Ⅰ interferon response- and interferon-gamma response-related genes in LM3 cells after NPL4 inhibition. ( L ) Heatmap of type Ⅰ interferon response-related genes in LM3 cells after NPL4 inhibition. *: p < 0.05; **, p < 0.01; ***, p < 0.001. The uncropped blots are shown in .

    Article Snippet: The supernatants of cells cultured under the conditions described above were evaluated for extracellular HMGB1 levels using an ELISA kit (Cusabio, CSB-E08223h and CSB-E08225m, Wuhan, China).

    Techniques: Inhibition, Western Blot, CCK-8 Assay, EdU Assay

    Figure 1. Schematic description of serum HMGB1 levels in patients and controls.

    Journal: Medicine

    Article Title: Is serum high-mobility group box 1 (HMGB-1) level correlated with liver fibrosis in chronic hepatitis B?

    doi: 10.1097/md.0000000000007547

    Figure Lengend Snippet: Figure 1. Schematic description of serum HMGB1 levels in patients and controls.

    Article Snippet: Serum HMGB1 levels were measured with ELISA assay csbe08223h© kit (Cusabio Biotech Co Ltd,Wuhan, Hubei Province, P.R.

    Techniques: